铁核桃离体培养与快速繁殖

以优良单株‘纳雍-1’的单芽茎段为外植体，建立了铁核桃（Juglanssigillata）离体培养与快速繁殖的体系。结果表明，附加6-BA1．0mg·L-1 ＋活·IgK（AC）3．0g·L-1的DKw培养基适宜铁核桃腋芽诱导；适宜铁核桃芽增殖的培养基为DKW＋6-BA1．0mg·L-1 ＋IBA0．02mg·L-1，40d后增殖系数可达7．33；试管苗的茎尖和茎段均可用于增殖培养；一步生根法（低浓度的生长素IBA持续诱导）不利于铁核桃试管苗嫩茎生根；采用二步生根法，生根率最高可达71．73％，其中，不同IBA浓度、暗培养时间、蔗糖浓度和AC含量对试管苗嫩茎生根影响显著，铁核桃试管苗在附]sulBA5,0mg·L-1的1／4DKW培养基中暗培养12d，再转移到不含IBA的1／4DKW培养基（附加AC 3g-L-1和蔗糖20g·L-1）中生根效果最好；生根试管苗采用珍珠岩和营养土两步炼苗，60d后成活率达到87．50％。

In Vitro Culture and Rapid Propagation of Juglans sigiilata

Single bud stem of superior individual Juglans sigillata cv. ＇Nayong-1＇ were used as explants to establish in vitro culture and rapid propagation system. Results showed that the DKW medium supplemented with 1.0 mg.L-1 6-BA＋3.0 g.L-1 AC was suitable for germination of axillary buds. The DKW medium with 1.0 mg.L-1 6-BA＋0.02 mg.L-1 IBA was optimal for the shoots multiplication, the proliferation coefficient was up to 7.33 after culturing for 40 days. Both shoot tip and single axillary bud could be used for multiplication culture. One step method （low IBA concentration sustained induction） was not suitable for rooting culture. The highest rooting rate （71.73%） was obtained by two steps method, firstly the plantlets was cultured on 1/4DKW medium with 5.0 mg.L-1 IBA in the dark condition for 12 days, and then transferred to the 1/4DKW medium without IBA under light （3 g.L-1 AC and 20 g.L-1 sucrose）. It was found that the IBA concentrations, time of dark induction, sucrose concentrations and AC contents were all related to the effect of rooting. After acclimation in pearlite and nutrient soil for 60 days, 87.50% plantlets were survival.