植物生长物质对大花红景天愈伤组织诱导及苗再生的影响

以大花红景天植物的茎、叶、芽、种子为材料,采用DPS软件正交设计法,通过添加不同植物生长物质,考察其对大花红景天愈伤组织形成及苗再生的影响,为低海拔条件下建立大花红景天植株再生体系提供数据基础。结果表明：种子和芽是诱导愈伤组织最适宜的外植体;植物生长物质对愈伤组织形成的影响大小为6-BA〉KT〉2,4-D〉NAA〉IAA,愈伤组织形成的最佳培养基为MS＋6-BA 4.0 mg.L-1＋KT 0.1 mg.L-1＋NAA 0.1 mg.L-1＋IAA 0.2 mg.L-1;不定芽分化的最佳培养基为MS＋TDZ 0.5 mg.L-1＋NAA 0.5 mg.L-1,诱导率达63.14%,不定芽数平均为11.4。本试验获得的最佳培养基配方,适宜在低海拔条件下进行快速诱导大花红景天的愈伤组织和植株再生。

Effects of Plant Growth Substances on Callus Induction and Shoot Regeneration of Rhodiola crenulata Hook. f. et Thoms.

To investigate the effects of different plant growth substances on callus induction and shoot regeneration of Rhodiola crenulata,the infancy stems,leaves,buds and seeds were chosen as test materials.The optimum combination of plant growth substances in MS solid medium for plant regeneration was established by using DPS software designing the orthogonal tests.The results showed that the seeds and buds were the optimal explants;the effect of plant growth substances on callus formation was 6-BA＆gt;KT＆gt;2,4D＆gt;NAA＆gt;IAA,the most effective medium was MS＋6-BA 4.0 mg.L-1＋KT 0.1 mg.L-1＋NAA 0.1 mg.L-1＋IAA 0.2 mg.L-1;the most appropriate medium for shoot regeneration was MS＋TDZ 0.5 mg.L-1＋NAA 0.5 mg.L-1,the induction rate was 63.14%,and the average number of adventitious bud was 11.4.The optimum combinations of plant growth substances in MS solid medium which were investigated in this project were avail to induce the callus and establish shoot regeneration system quickly in low altitude conditions.