| 玉米CMS-C不育系及其保持系线粒体膜通透性的比较分析 |
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| 引用本文： | 汪静,徐浩,王继玥,张艳花,荣廷昭,曹墨菊. 玉米CMS-C不育系及其保持系线粒体膜通透性的比较分析[J]. 植物生理学通讯, 2014, 0(6): 823-828 |
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| 作者姓名： | 汪静 徐浩 王继玥 张艳花 荣廷昭 曹墨菊 |
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| 作者单位： | 四川农业大学玉米研究所／农业部西南玉米生物学与遗传育种重点实验室,成都611130 |
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| 基金项目： | 四川农业大学学科建设双支计划. |
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| 摘 要： | 为了解玉米CMS-C相关不同育性材料雄穗线粒体膜通透性表现特征, 本文以玉米细胞质雄性不育系C48-2、保持系N48-2和育性恢复F1 （C48-2×18-599白）雄穗为材料, 测定了线粒体膜相关生理指标。结果发现, 从花粉母细胞时期到双核期C48-2雄穗线粒体膜吸光度、膜电位荧光强度、Ca2＋含量和Cyt c/a比值下降, MDA含量上升, 且各指标均在单核期和双核期与N48-2和F1 （C48-2×18-599白）有显著差异。
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| 关 键 词： | 玉米 CMS 线粒体 膜通透性 |
Comparative Analysis of Mitochondrial Membrane Permeability between Maize CMS-C Sterile Line and Its Maintainer Line |
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| WANG Jing,XU Hao,WANG Ji-Yue,ZHANG Yan-Hua,RONG Ting-Zhao,CAO Mo-Ju. Comparative Analysis of Mitochondrial Membrane Permeability between Maize CMS-C Sterile Line and Its Maintainer Line[J]. Plant Physiology Communications, 2014, 0(6): 823-828 |
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| Authors: | WANG Jing XU Hao WANG Ji-Yue ZHANG Yan-Hua RONG Ting-Zhao CAO Mo-Ju |
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| Affiliation: | ( Maize Research Institute of Siehuan Agricultural University Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Ministry of Agriculture, Chengdu 611130, China) |
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| Abstract: | In this study, in order to investigate the performance characteristic of mitochondrial membrane per meability in different fertility materials related to maize CMS-C, the physiological indexes of mitochondrial membrane were determined by using the tassels of sterile line C48-2, maintainer line N48-2 and fertility re stored F1 （C48-2x 18-599 white） as materials. The results indicated that membrane absorbance, membrane po- tential fluorescence intensity, Ca2＋ content and Cyt c/a ratio decreased, but MDA content increased in the mitochondria of C48-2 tassels, and these indexes were significantly different with those of N48-2 and F1 （C48-2＋ 18- 599 white） at uninucleate stage and binucleate stage. |
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| Keywords: | maize CMS （cytoplasmic male sterility） mitochondrion membrane permeability |
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