Variations des zymogrammes du tube digestif et du muscle chez Cyclonassa neritea en fonction de la température d'acclimatation |
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Authors: | Joel Trellu Hubert J Ceccaldi Henri Masse |
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Institution: | a Ecole Pratique des Hautes Etudes, Laboratoire de Biochimie et Ecologie des Invertébrés marins, Station marine d'Endoume, 13007, Marseille, France b Station marine d'Endoume, 13007, Marseille, France |
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Abstract: | Soluble proteins, esterases 2C, acid phosphatases of the digestive gland and foot muscle of Cyclonassa neritea, were compared using polyacrylamide gradient gels. α-Glucosidases, alkaline phosphatases, l-leucine aminopeptidase and peptidase were studied from digestive gland extracts. Molecular weights of isoenzymes were evaluated with 5000 d accuracy. Variation in activity of the most important isoenzymes of each enzyme under the influence of acclimation temperature was measured. In both muscle and digestive gland, the concentration of soluble proteins is stable. Through the whole acclimation temperature range, esterase activity per mg protein decreased with increased temperature. l-Leucine aminopeptidase activity decreases steadily from 10 to 25°, even though the two alkaline phosphatase isoenzyme activities increase. The other enzymes have their maximum activities at 20°. |
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Keywords: | Nassaridae Gastropoda Mollusca digestive gland muscle zymograms temperature |
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