Characterization of the superoxide anion radical scavenging activity by tetracycline antibiotics in aprotic media |
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Authors: | Aleksandra Kładna Irena Kruk Teresa Michalska Paweł Berczyński Hassan Y. Aboul‐Enein |
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Affiliation: | 1. Department of Medical History and Ethics, Pomeranian Medical University of Szczecin, , 70–204 Szczecin, Poland;2. Institute of Physics, Faculty of Mechanical Engineering and Mechatronics, West Pomeranian University of Technology in Szczecin, , 70–311 Szczecin, Poland;3. Pharmaceutical and Medicinal Chemistry Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, , Cairo, 12311 Egypt |
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Abstract: | The tetracycline family antibiotics are widely used as human and veterinary treatments. The drugs are effective as antibiotics and also show antimicrobial and non‐microbial action. However, the antioxidant properties of tetracyclines have not been characterized in aprotic media. To better understand their biological functions, the in vitro superoxide anion radical () scavenging activities of tetracycline, chlortetracycline, oxytetracycline, doxycycline and methacycline were characterized, along with a very efficient scavenger, tiron, in dimethyl sulphoxide (DMSO), using ultra‐weak chemiluminescence (CL). We found that tetracycline, chlortetracycline and doxycycline efficiently inhibited CL from the ‐generating system at concentration levels of 0.02–1.0 mmol/L. Methacycline and oxytetracycline were the scavengers at concentration levels of 0.01–0.1 mmol/L, whereas when their concentration was lowered the drugs were capable of generating , leading to CL enhancement. For all the data obtained in this study, the scavenging activity for the compounds tested decreased in the following order: tetracycline > doxycycline > chlortetracycline > tiron methacycline > oxytetracycline. These results indicate that the tetracycline drugs directly alter redox chemistry in aprotic media. Copyright © 2011 John Wiley & Sons, Ltd. |
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Keywords: | tetracyclines superoxide anion radical radical scavenging assay chemiluminescence |
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