In vitro cell culture pO2 is significantly different from incubator pO2 |
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| Authors: | L. L. Bambrick G. Rao |
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| Affiliation: | 1. Dept. of Anesthesiology, University of Maryland School of Medicine, Baltimore. MD 21201;2. Center for Advanced Sensor Technology, Dept. of Chemical and Biochemical Engineering, University of Maryland Baltimore County, Baltimore, MD 21250Conflicts of interest: G.R. and Y.K. have an equity position in Fluorometrix, Corporation |
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| Abstract: | Continuous noninvasive monitoring of peri‐cellular liquid phase pO2 in adherent cultures is described. For neurons and astrocytes, this approach demonstrates that there is a significant difference between predicted and observed liquid phase pO2. Particularly at low gas phase pO2s, cell metabolism shifts liquid phase pO2 significantly lower than would be predicted from the O2 gas/air equilibrium coefficient, indicating that the cellular oxygen uptake rate exceeds the oxygen diffusion rate. The results demonstrate the need for direct pO2 measurements at the peri‐cellular level, and question the widely adopted current practice of relying on setting the incubator gas phase level as means of controlling pericellular oxygen tension, particularly in static culture systems that are oxygen mass transfer limited. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011 |
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| Keywords: | oxygen neurons astrocytes sensor adherent cells cell culture |
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