Gold nanoparticles‐based fluorescence enhancement of the terbium–levofloxacin system and its application in pharmaceutical preparations |
| |
Authors: | Sang Hak Lee Saikh Mohammad Wabaidur Zeid Abdullah Alothman Seikh Mafiz Alam |
| |
Affiliation: | 1. Department of Chemistry, Kyungpook National University, , Taegu, 702‐701 Korea;2. Department of Chemistry, College of Science, King Saud University, , Riyadh, 11451 Kingdom of Saudi Arabia;3. Department of Chemistry, Aliah University, , Salt Lake City, Kolkata‐91, India |
| |
Abstract: | A sensitive fluorescence (FL) technique is proposed for the determination of levofloxacin (LVX). The method is based on the fact that the weak FL signal of the Tb(III)–LVX system is strongly enhanced in the presence of gold nanoparticles. Gold nanoparticles were prepared by the citrate reduction of HAuCl4 and characterized by transmission electron microscopy (TEM). Levofloxacin and Tb(III) ion form a fluorescence complex in aqueous solution, and its maximum emission wavelength was found at 545 nm. Optimal conditions for the formation of the levofloxacin–Tb(III) complexes were studied. Levofloxacin was detected by measuring the FL intensity, which increases linearly with the concentration of LVX in the range 6.2 × 10−10–2.6 × 10−8 mol/L. Recovery of the target analytes was > 96% with good quality parameters: linearity (r2 > 0.996), limit of detection (LOD) and limit of quantification (LOQ) values 2.1 × 10−10 mol/L and 7.2 × 10−10 mol/L, and run‐to‐run and day‐to‐day precisions with relative standard deviations (RSDs) around 3%. Thus, the proposed method can be successfully applied to the routine determination of levofloxacin in pharmaceutical preparations. Copyright © 2011 John Wiley & Sons, Ltd. |
| |
Keywords: | fluorescence levofloxacin terbium gold nanoparticles tablets |
|
|