Direct chemiluminescence determination of penicillin G potassium and a chemometrical optimization approach |
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Authors: | Mohammad H Sorouraddin Mortaza Iranifam Abdolhossein Naseri Mohammad Fadakar‐Sardroud Hossein Gharari‐Alibabalou |
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Institution: | 1. Analytical Chemistry Department, Faculty of Chemistry, University of Tabriz, , Iran;2. Department of Chemistry, Faculty of Science, University of Maragheh, , Maragheh, Iran |
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Abstract: | A highly selective and simple chemiluminescence (CL) method for determination of penicillin G potassium (PGK) was developed. In the proposed method, CL was elicited from PGK upon its oxidation with H2O2. The light emission was enhanced in the presence of N‐cetyl‐N,N,N‐trimethylammonium bromide (CTMAB). An experimental design, central composite design (CCD), was used to realize the optimized variables, including pH, surfactant (CTMAB) and H2O2 concentrations. Under optimum condition, the calibration graph was linear in the range 3.3 × 10?3–3.3 × 10?1 mmol/L, with a detection limit of 8.8 × 10?4 mmol/L for PGK. The precision was calculated by analysing samples containing 1.6 × 10?1 mmol/L PGK (n = 5) and the relative standard deviation (RSD) was 1.40%. The utility of this method was demonstrated by determining PGK in pharmaceutical formulations for injection. The proposed method was validated by a reference method. Copyright © 2011 John Wiley & Sons, Ltd. |
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Keywords: | chemiluminescence penicillin G potassium central composite design CTMAB H2O2 |
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