Fluorescence probe enhanced spectrofluorimetric method for the determination of sparfloxacin in tablets and biological fluids |
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Authors: | Akram M. El‐Didamony |
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Affiliation: | Department of Chemistry, Faculty of Science, Zagazig University, Zagazig, Egypt |
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Abstract: | Yttrium‐sensitized fluorescence was used to develop a sensitive and simple spectrofluorimetric method for the determination of sparfloxacin. The method is based on the strong fluorescence of sparfloxacin after adding the fluorescence probe yttrium in buffer solution (pH = 8), and various factors influencing fluorescence were investigated. Under optimum conditions, the enhanced fluorescence intensity of the system showed a good linear relationship with the concentration of sparfloxacin over the range 8 × 10?7 to 1.4 × 10?5 mol L?1 with a correlation coefficient of 0.9997. The detection limit (S/N = 3) was determined as 9.01 × 10?8 mol L?1. The mechanism of the sensitizing effect of probe was discussed. This method has been successfully applied for the determination of sparfloxacin in pharmaceuticals, human urine and serum samples; the result obtained was satisfactory. Copyright © 2010 John Wiley & Sons, Ltd. |
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Keywords: | sparfloxacin yttrium spectrofluorometry tablets urine serum |
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