Fluorescence probe enhanced spectrofluorimetric method for the determination of sparfloxacin in tablets and biological fluids

Yttrium‐sensitized fluorescence was used to develop a sensitive and simple spectrofluorimetric method for the determination of sparfloxacin. The method is based on the strong fluorescence of sparfloxacin after adding the fluorescence probe yttrium in buffer solution (pH = 8), and various factors influencing fluorescence were investigated. Under optimum conditions, the enhanced fluorescence intensity of the system showed a good linear relationship with the concentration of sparfloxacin over the range 8 × 10^?7 to 1.4 × 10^?5 mol L^?1 with a correlation coefficient of 0.9997. The detection limit (S/N = 3) was determined as 9.01 × 10^?8 mol L^?1. The mechanism of the sensitizing effect of probe was discussed. This method has been successfully applied for the determination of sparfloxacin in pharmaceuticals, human urine and serum samples; the result obtained was satisfactory. Copyright © 2010 John Wiley & Sons, Ltd.