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Rate constants for the hexamer-dodecamer reaction of lobster hemocyanin
Authors:Gerson Kegeles  Mei-Sheng Tai
Affiliation:Section of Biochemistry and Biophysics, University of Connecticut, Storrs, Connecticut 06268, USA
Abstract:90° stopped-flow light scattering experiments have been used to determine the forward and reverse rate constants of the reversible hexamer-dodecamer reaction of lobster hemocyanin in glycine buffer at pH 9.6. Results were obtained at 20.5° and 25° at two different levels of calcium ion, and equilibrium constants derived from the data are in good agreement with those measured earlier with the ultracentrifuge.
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