Chromatographic separation and spectroscopic characterization of the e/z isomers of acrivastine |
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| Authors: | Prakash M Davadra Batuk Dabhi Manoj K Singh Mukul R Jain Hitendra S Joshi Atul H Bapodra |
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| Institution: | 1. Email:prakashdavadra@zyduscadila.com or prakashdavadra@yahoo.co.in;4. Department of Biopharmaceutics, Zydus Research Centre, Ahmedabad, Gujarat, India;5. Department of Strategic Technology Development, Zydus Research Centre, Ahmedabad, Gujarat, India;6. Department of Pharmacology and Toxicology, Zydus Research Centre, Ahmedabad, Gujarat, India;7. Department of Chemistry, Saurashtra University, Rajkot, Gujarat, India;8. Department of Chemistry, M. D. Science College, Porbandar, Gujarat, India |
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| Abstract: | A reverse phase high performance liquid chromatography (HPLC) method has been developed for the separation of two geometric isomers of Acrivastine using crude reaction mixture. The resolution between two isomers was found more than 2.9. The geometric isomers have been isolated by preparative HPLC and characterized by spectroscopic techniques, such as NMR, infrared, and MS. The developed method has been validated for the determination of Z‐isomer in Acrivastine. The limit of detection and limit of quantification of the Z‐isomer were 0.05 and 0.2 μg/ml, respectively. The developed method is precise, linear, accurate, rugged and robust for its intended use. Chirality, 2011. © 2011 Wiley‐Liss, Inc. |
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| Keywords: | Acrivastine geometric isomers E/Z isomers HPLC validation |
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