Bacillus subtilis pgsE (Formerly ywtC) stimulates poly‐γ‐glutamate production in the presence of zinc |
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| Authors: | Daisuke Yamashiro Megumi Yoshioka Makoto Ashiuchi |
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| Institution: | 1. Course of Applied Bioresource Science, United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime, Japan;2. Agricultural Science, Graduate School of Integrated Arts and Sciences, Kochi University, Nankoku, Kochi 783‐8502, Japan;3. telephone: +81‐88‐864‐5215;4. fax: +81‐88‐864‐5200 |
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| Abstract: | Poly‐γ‐glutamate (PGA) is a versatile nylon‐like material, and enhanced production of PGA is required for various bio‐industrial applications. In this study, we first examined the effects of available sugars on the production of Bacillus subtilis PGA, and demonstrated the good applicability of pentoses (e.g., D ‐xylose). Then, we characterized the pgsE gene of B. subtilis, which encodes a 6.5‐kDa protein of 55 amino acids (PgsE), as a genetic tool for increasing the yield of PGA without changing its structural features (e.g., polymer stereochemistry and molecular size distribution). In the presence of Zn2+, the induction of PgsE tripled the PGA productivity of B. subtilis subsp. chungkookjang. This finding will contribute to the establishment of an improved PGA‐production system. Biotechnol. Bioeng. 2011; 108:226–230. © 2010 Wiley Periodicals, Inc. |
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| Keywords: | poly‐γ ‐glutamate Bacillus subtilis genetic manipulation pgsE zinc ion |
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