| 白色念珠菌RAPD反应体系优化的研究 |
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| 引用本文： | 厉荣玉,钱森和,王萍,董群.白色念珠菌RAPD反应体系优化的研究[J].生物学杂志,2010,27(1):39-42. |
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| 作者姓名： | 厉荣玉 钱森和 王萍 董群 |
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| 作者单位： | 1. 皖南医学院微生物学与免疫学教研室,芜湖,241001
2. 安徽工程科技学院生化系,芜湖,241000 |
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| 基金项目： | 皖南医学院中青年科研基金--不同基因型念珠菌感染临床株的分析 |
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| 摘 要： | 建立白色念珠菌RAPD的最佳反应体系，并应用于其基因组DNA扩增。通过单因子试验分别研究了Mg^2＋、dNTPs、Taq酶、引物和模板DNA等浓度对RAPD反应的影响；同时，应用L16（4^5）正交试验研究了DNA模板、Mg^2＋、Taq酶、dNTPs和引物浓度对RAPD反应的影响。以条带稳定、丰富、清晰为标准，获得了白色念珠菌基因组DNA的RAPD扩增优化条件；对于白色念珠菌的最适RAPD反应体系为Mg^2＋1．5mmol／L、dNTPs250μmol／L、引物0．6μmol／L、模板100ng／25μL、TaqDNA聚合酶1．5U／25μL。
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| 关 键 词： | 白色念珠菌 RAPD反应体系 优化 |
Studies on optimization of RAPD reaction system for Candidia Albicans |
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| LI Rong-yu,QIAN Sen-he,WANG Ping,DONG Qua.Studies on optimization of RAPD reaction system for Candidia Albicans[J].Journal of Biology,2010,27(1):39-42. |
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| Authors: | LI Rong-yu QIAN Sen-he WANG Ping DONG Qua |
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| Institution: | LI Rong-yu1,QIAN Sen-he2,WANG Ping1,DONG Qun1(1.Department of Microbiology , Immunology,Wannan Medical College,Wuhu 24001,2.Department of Biochemistry,Anhui University of Technology , Science,Wuhu 241000,China) |
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| Abstract: | To establish the optimization of RAPD reaction system for Candidia Albicans,the genomic DNA amplification was used for test.Effects of the concentrations of different substances like Mg2+,dNTPs,DNA,primer,and Taq polymerase on RAPD amplification were studied through single factor experimentation.At the same time,the L16(45) orthogonal experimentation was applied to study the effects of different concentrations of DNA,Mg2+,Taq polymerase,dNTPs,and primer.Result showed the optimization of RAPD reaction system... |
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| Keywords: | Candida albicans RAPD reaction system optimization |
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