Cyclic AMP-dependent protein kinase of Neurospora crassa |
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Authors: | P A Powers M L Pall |
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Affiliation: | 1. Program in Genetics Washington State University, Pullman, WA 99164 U.S.A.;2. Program in Biochemistry and Biophysics Washington State University, Pullman, WA 99164 U.S.A. |
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Abstract: | was surveyed for cyclic AMP-dependent protein kinase activity. Two peaks (I and II) of protein kinase activity were demonstrated by DEAE-cellulose chromatography of wild type extracts. Peak I was stimulated by cyclic AMP, eluted below 60 mM NaCl and had high activity using histone H2B as substrate. Peak II eluted at 200–250 mM NaCl; its activity was not cyclic AMP stimulated and was highest with dephosphorylated casein as a substrate. Cyclic AMP binding to a protein associated with the protein kinase is specifically inhibited by certain cyclic AMP analogs. |
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Keywords: | To whom correspondence should be addressed. |
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