The intrahepatic immune response during chronic hepatitis B infection can be monitored by the fine-needle aspiration biopsy technique |
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Authors: | Tang Thjon J Janssen Harry L A Kusters Johannes G de Man Robert A Schalm Solko W Kwekkeboom Jaap |
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Affiliation: | Department of Gastroenterology and Hepatology, Room L-455, Erasmus MC, University Medical Center Rotterdam, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands. |
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Abstract: | Frequent analysis of the intrahepatic cellular immune response during chronic hepatitis B infection is not feasible with the liver tissue biopsy technique, due to its risk profile and patient discomfort. We investigated whether the relatively safe and patient-friendly cytological fine-needle aspiration biopsy (FNAB) technique is suited for this purpose. FNABs taken during hepatitis flares in three chronic hepatitis B patients treated with interferon-alpha, showed significant increments of CD8(+)-lymphocytes compared with the FNABs taken before and after the flares. No increments were observed in peripheral blood. The increments of intrahepatic CD8+ lymphocytes detected by the FNAB were related to anti-viral immune reactivity, since they coincided with significant serum hepatitis B virus DNA level reductions and in two of three patients with HBeAg seroconversion. In conclusion, the FNAB technique is suited to investigate the intrahepatic immune response during chronic hepatitis B infection on a frequent basis. |
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Keywords: | Fine-needle aspiration biopsy Chronic hepatitis Hepatitis B virus Immunocytochemistry Anti-viral therapy CD8+ T-lymphocyte |
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