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Detection of bovine herpesvirus 1 and 5 in semen from Brazilian bulls |
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| Authors: | Oliveira M T Campos F S Dias M M Velho F A Freneau G E Brito W M E D Rijsewijk F A M Franco A C Roehe P M |
| Institution: | a Virology Laboratory, Microbiology Department, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Av. Sarmento Leite 500, Porto Alegre, CEP 90050-170, Rio Grande do Sul (RS), Brazil b Virology Laboratory, Microbiology Department, Federal University of Goiás (UFG), Rod GO 080 Q Área, s/n lt 1. Campus Universitário, Goiânia, CEP 74001-970, Goiás (GO), Brazil c FEPAGRO, Animal Health, Institute for Veterinary Research “Desidério Finamor” (IPVDF), Estrada do Conde 6000, Eldorado do Sul, CEP 92990-000, Rio Grande do Sul (RS), Brazil |
| Abstract: | Bovine herpesvirus 1 (BoHV-1) and 5 (BoHV-5) are important pathogens of the respiratory and genital tract of cattle and may also affect the central nervous system and cause meningoencephalitis. Both virus types are estimated to be widely distributed in Southern Brazil. In the present study, BoHV-1 and/or BoHV-5 DNA were detected in bovine semen samples from two states of Brazil by two species-specific nested polymerase chain reactions (nPCRs). These nPCRs were used to assay 53 samples of fresh semen and 23 samples of frozen semen from breeding bulls. Viral DNA was detected in all 76 semen samples: all were positive for BoHV-5, whereas 34 of these were positive for BoHV-1 as well. Moreover, in five fresh and in 13 frozen semen samples—of a total number of 40 samples suitable for virus isolation—infectious BoHV-1 and/or BoHV-5 virus were detected. In conclusion, that both BoHV-1 and BoHV-5 were detected in bovine semen in Brazil highlighted the importance of examining bull semen in search for both agents to reduce the risk of transmitting these viruses. |
| Keywords: | BoHV-1 BoHV-5 Bull semen Herpesvirus Nested PCR |
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