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Determination of glutation peroxidase and superoxide dismutase activities in canine seminal plasma and its relation with sperm quality and lipid peroxidation post thaw |
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| Authors: | VR Neagu B Macías GarcíaA Morillo Rodríguez C Ortega FerrusolaJM Gallardo Bolaños L González FernándezJA Tapia FJ Peña |
| Affiliation: | a Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Avd de la Universidad s/n, Cáceres, Spain b Department of Physiology Faculty of Veterinary Medicine, University of Extremadura, Avd de la Universidad s/n, Cáceres, Spain c Department of Obstetrics Gynecology and Reproduction Faculty of Veterinary Medicine University of Agricultural Sciences and Veterinary Medicine Calea Manastur 3-5 Cluj Napoca 400372 Romania |
| Abstract: | Lipid peroxidation (LPO) of dog spermatozoa was assessed in fresh semen and in samples of the same ejaculates after freezing and thawing. Particular attention was paid to individual differences in the susceptibility to LPO and its possible relationship with freezeability. Innate levels of LPO were low in fresh spermatozoa but increased after thawing in one of the dogs included in our study. The level of lipid peroxidation in fresh spermatozoa was not correlated with that of thawed spermatozoa. Negative correlations were detected between the activity in seminal plasma of GPx and sperm velocities post thaw (P < 0.01), however SOD activity was positively correlated with the percentage of linear motile sperm post thaw (P < 0.05). |
| Keywords: | Spermatozoa Lipid peroxidation Freezing Dog Flow cytometry |
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