Effectiveness of slow freezing and vitrification for long-term preservation of mouse ovarian tissue |
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Authors: | Kim G A Kim H Y Kim J W Lee G Lee E Ahn J Y Park J H Lim J M |
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Affiliation: | a Department of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Korea b Dental Research Institute, School of Dentistry, Seoul National University, Seoul 110-749, Korea c School of Veterinary Medicine, Kangwon National University, Chunchon 200-701, Korea d Laboratory of Stem Cell and Bioevaluation, WCU Biomodulation Program, Seoul National University, Seoul 151-742, Korea |
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Abstract: | This study was conducted to evaluate the interaction between cryo-damage and ART outcome after cryopreservation of mouse ovarian tissues with different methods. Either a vitrification or a slow freezing was employed for the cryopreservation of B6CBAF1 mouse ovaries and follicle growth and the preimplantation development of intrafollicular oocytes following parthenogenesis or IVF were monitored. Both cryopreservation protocols caused significant damage to follicle components, including vacuole formation and mitochondrial deformities. Regardless of the cryopreservation protocols employed, a sharp (P < 0.0001) decrease in follicle viability and post-thaw growth was detected. When IVF program was employed, significant (P < 0.05) decrease in cleavage and blastocyst formation was notable in both modes of cryopreservation. However, such retardation was not found when oocytes were parthenogenetically activated. In the IVF oocytes, slow freezing led to better development than vitrification. In conclusion, a close relationship between cryopreservation and ART methods should be considered for the selection of cryopreservation program. |
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Keywords: | Ovarian follicle Slow freezing Vitrification IVF Parthenogenesis Preimplantation development |
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