The FOX hunting system: an alternative gain-of-function gene hunting technique |
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Authors: | Ichikawa Takanari Nakazawa Miki Kawashima Mika Iizumi Haruko Kuroda Hirofumi Kondou Youichi Tsuhara Yumi Suzuki Kumiko Ishikawa Akie Seki Motoaki Fujita Miki Motohashi Reiko Nagata Noriko Takagi Tomoko Shinozaki Kazuo Matsui Minami |
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Institution: | Plant Functional Genomics Research Team, Plant Functional Genomics Research Group, Plant Science Center RIKEN Yokohama Institute, 1-7-22 Suehirocho, Tsurumiku, Yokohama, Kanagawa, 230-0045, Japan. |
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Abstract: | We have developed a novel gain-of-function system that we have named the FOX hunting system (Full-length cDNA Over-eXpressing gene hunting system). We used normalized full-length cDNA and introduced each cDNA into Arabidopsis by in planta transformation. About 10 000 independent full-length Arabidopsis cDNAs were expressed independently under the CaMV 35S promoter in Arabidopsis. Each transgenic Arabidopsis contained on average 2.6 cDNA clones and was monitored under various categories such as morphological changes, fertility and leaf color. We found 1487 possible morphological mutants from 15 547 transformants. When 115 pale green T(1) mutants were analyzed, 59 lines represented the mutant phenotypes in more than 50% of the T(2) progeny. Characterization of two leaf color mutants revealed the significance of this approach. We also document mutants from several categories and their corresponding full-length cDNAs. |
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Keywords: | Functional genomics full-length cDNA dominant phenotype unknown gene pale green Arabidopsis |
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