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Drosophila genome project: One-hit coverage in yeast artificial chromosomes |
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| Authors: | James W Ajioka David A Smoller Robert W Jones John P Carulli Ann E C Vellek Dan Garza Andrew J Link Ian W Duncan Daniel L Hartl |
| Institution: | (1) Department of Genetics, Washington University School of Medicine, 63110-1095 St. Louis, MO, USA;(2) Department of Genetics, Harvard Medical School, 02115 Boston, MA, USA;(3) Department of Biology, Washington University, 63130 St. Louis, MC, USA;(4) Present address: London School of Hygiene and Tropical Medicine, London, UK;(5) Present address: Department of Development Biology, Stanford University, Stanford, CA, USA |
| Abstract: | We present a strategy for assembling a physical map of the genome of Drosophila melanogaster based on yeast artificial chromosomes (YACs). In this paper we report 500 YACs containing inserts of Drosophila DNA averaging 200 kb that have been assigned positions on the physical map by means of in situ hybridization with salivary gland chromosomes. The cloned DNA fragments have randomly sheared ends (DY clones) or ends generated by partial digestion with either NotI (N clones) or EcoRI (E clones). Relative to the euchromatic portion of the genome, the size distribution and genomic positions of the clones reveal no significant bias in the completeness or randomness of genome coverage. The 500 mapped euchromatic clones contain an aggregate of approximately 100 million base pairs of DNA, which is approximately one genome equivalent of Drosophila euchromatin.by W. Hennig |
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