Endogenous 15-deoxy-Delta(12,14)-prostaglandin J(2) synthesized by adipocytes during maturation phase contributes to upregulation of fat storage |
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Authors: | Mazid Md Abdul Chowdhury Abu Asad Nagao Kohjiro Nishimura Kohji Jisaka Mitsuo Nagaya Tsutomu Yokota Kazushige |
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Affiliation: | Department of Life Science and Biotechnology, Shimane University, Matsue, Shimane 690-8504, Japan. |
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Abstract: | 15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) has been identified as a natural ligand for peroxisome proliferator-activated receptor (PPAR) γ to promote adipogenesis. However, it remains elusive about the ability of PPARγ-expressing adipocytes to produce PGJ2 series and the role in the life cycle of adipocytes. Here, we developed an enzyme-linked immunosorbent assay specific for 15d-PGJ2. The analysis using this method revealed the increase in the endogenous synthesis of immunoreactive 15d-PGJ2 in cultured adipocytes during the maturation phase. Further studies using cyclooxygenase inhibitors clarified the contribution of endogeous 15d-PGJ2 produced by mature adipocytes to upregulation of fat storage in an autocrine manner. |
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Keywords: | Prostaglandin D2 15-Deoxy-Δ12,14-prostaglandin J2 Cyclooxygenase Adipocyte Adipogenesis Peroxisome proliferator-activated receptor γ |
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