| Abstract: | The effect of the culture medium composition on the bacitracin synthesis and sporulation in Bacillus licheniformis 28 KA was being studied. During the producer's growth on a medium with tripton a biphasic pattern of the process was observed, i.e. a rapid growth of the bacterial biomass was accompanied by the production of insignificant amounts of the antibiotic. The maximum efficiency of the antibiotic synthesis coincides with the end of the exponential growth phase and the onset of sporification. The efficiency of sporification amounts up to 95% when the above mentioned medium is used. The substitution of glutamate for trypton results in a dramatic deceleration of the bacterial growth and biomass accumulation, and the process of the antibiotic biosynthesis ceases to be biphasic, i. e. the antibiotic is synthesized during the trophophase. Under the conditions when the antibiotic production drops to zero by the middle of the exponential growth phase, sporulation is virtually suppressed. |
