Establishment and characterization of insect cell lines from 10 lepidopteran species |
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Authors: | Goodman C L El Sayed G N McIntosh A H Grasela J J Stiles B |
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Institution: | (1) Department of Entomology, University of Missouri, 65211 Columbia, Missouri;(2) Present address: BASF Agro Research American Cyanamid Co., Agricultural Research Div., P.O. Box 400 Princeton, New Jersey, 08540;(3) Biological Control of Insects Research Laboratory, U.S. Department of Agriculture, Agricultural Research Service, 1503 S. Providence Road, 65203-3535 Columbia, Missouri |
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Abstract: | Summary Cell lines from selected lepidopteran species were established for the overall purpose of use in baculovirus production. A
total of 36 new cell lines from 10 lepidopteran species were generated, including cell lines from a pyralid, the European
corn borer,Ostrinia nubilalis, a plutellid, the diamondback moth,Plutella xylostella, as well as eight noctuids: the black cutworm,Agrotis ipsilon, the celery looper,Anagrapha falcifera, the velvetbean caterpillar,Anticarsia gemmatalis, the corn earworm,Helicoverpa zea, the tobacco budworm,Heliothis virescens, the beet armyworm,Spodoptera exigua, the fall armyworm,Spodoptera frugiperda, and the cabbage looper,Trichoplusia ni. Tissues used for cell line establishment included fat bodies, ovaries, testes, or whole embryos/larvae/pupae. All the cell
lines were subcultured numerous times, characterized by isoenzyme analysis and/or deoxyribonucleic acid amplification fingerprinting
using polymerase chain reaction, and stored in liquid nitrogen. Many of the cell lines were adapted to grow in serum-free
medium, with cell lines fromA. ipsilon andH. virescens being adapted to suspension culture, using shaker flasks. The potential use for these cell lines in baculovirus production
is discussed.
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Keywords: | DNA fingerprinting isoenzymes baculovirus noctuidae plutellidae pyralidae |
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