The relation between sphingomyelinase activity, lipid peroxide oxidation and NO-releasing in mice liver and brain |
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| Authors: | Alessenko A V Shupik M A Bugrova A E Dudnik L B Shingarova L N Mikoyan A Vanin A F |
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| Affiliation: | Institute of Biochemical Physics of the Russian Academy of Sciences, Moscow. ales@sky.chph.ras.ru |
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| Abstract: | We used animal models to study connection between oxidating system and sphingomyelin signaling cascade, because this models are more close related to people disease. Activation of n-sphingomyelinase (n-SMase) in mice liver and brain is coincided in time with increased level of peroxide products (conjugated dienes) after injection of tumor necrosis factor alpha (TNF-alpha). We found that ceramide can induce peroxide oxidation and lead to accumulation of TNF-alpha in animal organs. Nitric oxide (NO) donors (S-nitrosoglutathione and dinitrosyl iron complex) reversibly inhibited activity of n-SMase and decreased level of lipid peroxidation products. This data proposed that both SMase and messengers of oxidative systems could be targets for NO-derived oxidants. |
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| Keywords: | DNIC, dinitrosyl iron complex GSH, glutathione GSNO, S-nitrosoglutathione LPO, lipid peroxidation NO, nitric oxide n-SMase, n-sphingomyelinase ROS, reactive oxygen species SM, sphingomyelin TNF-α, tumor necrosis factor α |
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