多效调控基因degU32(Hy),degQa和degR对枯草芽孢杆菌Ki-2-132生长和蛋白酶发酵的影响

通过向枯草芽孢杆菌Ki-2-132染色体和／或细胞质导入来自枯草杆菌168菌株的degU32（Hy）和degR基因，以及来自芽孢杆菌解淀粉菌株（Bacillus amyloliquefaciens）的degQa基因，对上述基因对枯草芽孢杆菌Ki-2-132细胞的生长、孢子发生、蛋白酶发酵的影响进行了研究。尽管上述多效调控基因来自不同的芽孢杆菌种和菌株，它们在枯草芽孢杆菌Ki-2-132中依然表现多效性。枯草杆菌Ki-2-132degU32（Hy）表现出增高了的蛋白酶产量；当和质粒或染色体上的degQa基因协作，可以进一步依赖葡萄糖的水平和degQa的基因剂量影响细胞生长，增加蛋白酶产量，以及影响孢子的形成。与此不同，degR在degU32（Hy）突变体中并不显著影响其蛋白酶的产量，这一发现支持DegR蛋白通常稳定磷酸化的DegU，而其在degU32（Hy）菌株中不再进一步放大该突变体内已被磷酸化的DegU的调控作用。

Effects of degU32(Hy), degQa and degR Pleiotropic Regulatory Genes on the Growth and Protease Fermentation of Bacillus Subtilis Ki-2-132

Effects of degU32 (Hy), degR genes from Bacillus subtilis 168 and degQa gene from Bacillus amyloliquefaciens on Bacillus subtilis Ki-2-132 cell growth, sporulation and protease fermentation were investigated by introducing these genes into B. subtilis Ki-2-132 chromosome and/or cytoplasm. Although the genes come from different species and strains, they showed plei-otropic effects in B. subtilis Ki-2-132. B. subtilis Ki-2-132degU32 (Hy) showed increased protease production, and when cooperat-ing with degQa either in plasmid or in chromosome, further altered cell growth, increased protease production and affected the spore formation in a glucose and dosage dependent manner. By contrast, degR did not significantly affect the protease productivity in degU32 (Hy) mutant, consisting with that DegR was used to stabilise DegU-phosphate, which in degU32 (Hy) strain no longer further amplify the DegU-phosphate effect.