A comparative study of some properties of cytidine deaminase from Escherichia coli and chicken liver |
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| Authors: | A Vita T Cacciamani P Natalini S Ruggieri N Raffaelli G Magni |
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| Affiliation: | Dipartimento di Biologia Cellulare, Università di Camerino, Italy. |
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| Abstract: | The molecular and kinetic properties of cytidine deaminase from E. coli and chicken liver show several interesting differences and similarities: 1. Both enzymes possess an oligomeric structure, and linear kinetics. 2. The chicken liver enzyme is strictly dependent on the presence of reducing agents and presents a microheterogeneity in the pure preparation. 3. Both enzymes display identical specificity and share a rapid-equilibrium random Uni-Bi mechanism of catalysis. 4. The chicken liver enzyme is inhibited competitively by dTTP, CMP and dCMP. |
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