Calcium influx in a single rat basophilic leukemia cell as revealed with a digital imaging fluorescence microscope |
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Authors: | K Kato M Nakanishi Y Arata R Teshima T Terao H Miyamoto |
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Affiliation: | Faculty of Pharmaceutical Sciences, University of Tokyo. |
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Abstract: | Using a digital imaging fluorescence microscope, we have detected a rapid transient increase in the free cytosolic calcium concentration in a single rat basophilic leukemia cell (RBL-2H3) after antigen stimulation. Calcium ions were transported very rapidly (within 1 s) after a lag time (about 10 s at 37 degrees C) from the external environment into the cytoplasm. On the basis of the present experimental results we conclude that the gradual changes in the overall fluorescence intensity observed for a cell suspension are due to the distribution of different lag times shown by different cells as to the calcium influx through membrane calcium channels. |
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