Detection of B virus infection in cynomolgus monkeys by ELISA using simian agent 8 as alternative antigen. |
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| Authors: | J Takano T Narita K Fujimoto R Mukai A Yamada |
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| Institution: | Corporation for Production and Research of Laboratory Primates, 1 Hachimandai, Tsukuba-shi, Ibaraki 305-0843, Japan. |
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| Abstract: | The use of simian agent 8 (SA8) as an antigen for B virus (BV) antibody detection was evaluated in cynomolgus monkeys. Seventy-two sera judged as positive using BV antigen were all positive when the SA8 antigen was used. Out of 28 BV-negative sera 2 were positive against the SA8 antigen and one serum was classified as indeterminate. The present data indicates that detection of BV antibody can be achieved accurately and safely by enzyme-linked immunosorbent assay (ELISA) using SA8 antigen. |
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