Characterization of a Novel Phosphoinositide-Specific Phospholipase C
from <Emphasis Type="Italic">Zea mays</Emphasis> and its Expression in <Emphasis Type="Italic">Escherichia coli</Emphasis> |
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Authors: | Shumei?Zhai Zhenhua?Sui Aifang?Yang Email author" target="_blank">Juren?ZhangEmail author |
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Institution: | (1) School of Life Science, Shandong University, Jinan, 250100, P.R. China |
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Abstract: | A cDNA encoding a phosphoinositide-specific phospholipase C (PI-PLC) has been isolated from Zea mays by screening a cDNA library. The cDNA, designated ZmPLC, encodes a polypeptide of 586 amino acids, containing the catalytic X, Y and C2 domains found in all PI-PLCs from plants. Northern blot analysis showed that the expression of the ZmPLC gene in roots is up-regulated under conditions of high salt, dehydration, cold or low osmotic stress conditions. Recombinant ZmPLC protein was expressed in Esch- erichia coli, purified and used to produce polyclonal antibody, this polyclonal antibody is important for further studies to assess the ultimate function of the ZmPLC gene in plants. |
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Keywords: | cloning expression phospholipase C polyclonal antibody Zea mays |
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