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| 响应面法优化蕤核叶片总黄酮提取工艺 | |
| 引用本文： | 张秀秀,魏学智,张强,徐建国,邰源临. 响应面法优化蕤核叶片总黄酮提取工艺[J]. 中国野生植物资源, 2011, 30(2): 27-31. DOI: 10.3969/j.issn.1006-9690.2011.02.007 |
| 作者姓名： | 张秀秀 魏学智 张强 徐建国 邰源临 |
| 作者单位： | 1. 山西师范大学生命科学学院,山西临汾,041000 2. 山西师范大学工程学院,山西临汾,041000 3. 水利部水土保持植物开发管理中心,北京,100083 |
| 摘 要： | 采用超声波辅助提取的方法从蕤核叶片中提取总黄酮.利用响应面法(RSM法)研究了超声提取时间、乙醇浓度、液固比、提取温度等因素对总黄酮得率的影响,确定了超声波辅助提取蕤核叶片总黄酮的最佳工艺参数.结果表明,超声波辅助提取蕤核叶片总黄酮的最佳工艺务件为:超声时间50.1 min,乙醇浓度59.5%,液料比24.9:1,提取... |
| 关 键 词： | 蕤核 总黄酮 响应面法 提取工艺 超声波 |
Optimization of Extracting Total Flavonoids from Prinsepia uniflora Batal.Using Response Surface Methodology |
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| Zhang Xiuxiu,Wei Xuezhi,Zhang Qiang,Xu Jianguo,Tai Yuanlin. Optimization of Extracting Total Flavonoids from Prinsepia uniflora Batal.Using Response Surface Methodology[J]. Chinese Wild Plant Resources, 2011, 30(2): 27-31. DOI: 10.3969/j.issn.1006-9690.2011.02.007 | |
| Authors: | Zhang Xiuxiu Wei Xuezhi Zhang Qiang Xu Jianguo Tai Yuanlin |
| Affiliation: | 1. School of Life Science, Shanxi Normal University, Linfen 041000, China; 2. Project Academy, Shanxi Normal University, Linfen 041000, China; 3. Plant Development Center for Soil and Water Conservation, Ministry of Water Resources, Beijing 100083, China) |
| Abstract: | Total flavonoids were extracted from Prinsepia uniflora Batal. leaves by using ultrasonic method. Ultrasonic time, ethanol concentration, liquid -solid ratio and extraction temperature were selected as influencing factors during extraction, and the protocol of extracting total flavoniods from Prinsepia uniflora Batal. leaves was optimized by response surface methodology. The results showed that the optimum extraction conditions were： ultrasonic time 50.1 min, ethanol concentration 59.5%, liquid -solid ratio 24.9：1 and extraction temperature 72.8 ℃. The extraction rate was 1. 151 mg·g^-1 under these conditions. |
| Keywords: | Prinsepia uniflora Batal. total flavonoids response surface methodology extraction protocol ultrasound |
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