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High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage
Authors:Ian M Slaymaker  Pablo Mesa  Max J Kellner  Soumya Kannan  Edward Brignole  Jeremy Koob  Patricia R Feliciano  Stefano Stella  Omar O Abudayyeh  Jonathan S Gootenberg  Jonathan Strecker  Guillermo Montoya  Feng Zhang
Institution:1. Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA;2. McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;3. Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;4. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;5. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;6. Department of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;7. Department of Systems Biology, Harvard University, Boston, MA 02115, USA;8. Department for Biochemistry and Cell Biology, University of Vienna, Dr Bohr-Gasse 9, 1030 Vienna, Austria;9. Protein Structure & Function Programme, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, Structural Molecular Biology Group, University of Copenhagen, Copenhagen, Denmark
Abstract:
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  • Keywords:CRISPR-Cas9 system  Cas13b  type VI CRISPR  crystal structure  RNA-targeting
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