Discovery of highly reactive peptide tag by ELISA-type screening for specific cysteine conjugation |
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| Authors: | Nobutaka Kurashige Hirokazu Fuchida Shigekazu Tabata Shohei Uchinomiya Akio Ojida |
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| Institution: | Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan |
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| Abstract: | We report the discovery of a highly reactive peptide tag for the specific cysteine conjugation of proteins. Screening of cysteine-containing peptides using ELISA-type screening yielded a 19-amino acid tag (DCPPPDDAADDAADDAADD), named DCP3 tag, which enabled the rapid and selective labeling of the tag-fused protein with a synthetic zinc complex on the surface of living cells. |
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| Keywords: | Protein labeling Peptide tag ELISA Fluorescence imaging |
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