Ferrocene conjugated oligonucleotide for electrochemical detection of DNA base mismatch |
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| Authors: | Yusuke Hasegawa Tadao Takada Mitsunobu Nakamura Kazushige Yamana |
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| Affiliation: | 1. Department of Materials Science and Chemistry, University of Hyogo, 2167 Shosha, Himeji 671-2280, Japan;2. Department of Applied Chemistry, Graduate School of Engineering, University of Hyogo, 2167 Shosha, Himeji 671-2280, Japan |
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| Abstract: | We describe the synthesis, binding, and electrochemical properties of ferrocene-conjugated oligonucleotides (Fc-oligos). The key step for the preparation of Fc-oligos contains the coupling of vinylferrocene to 5-iododeoxyuridine via Heck reaction. The Fc-conjugated deoxyuridine phosphoramidite was used in the Fc-oligonucleotide synthesis. We show that thiol-modified Fc-oligos deposited onto gold electrodes possess potential ability in electrochemical detection of DNA base mismatch. |
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| Keywords: | Ferrocene Oligonucleotide Conjugate Electrochemical detection DNA mismatch |
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