Protective effect of Opuntia ficus-indica L. cladodes against UVA-induced oxidative stress in normal human keratinocytes |
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Authors: | Ganna Petruk Flaviana Di Lorenzo Paola Imbimbo Alba Silipo Andrea Bonina Luisa Rizza Renata Piccoli Daria Maria Monti Rosa Lanzetta |
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Institution: | 1. Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte Sant’Angelo, via Cinthia 4, 80126 Naples, Italy;2. Bionap srl, R&D Contrada Fureria, ZIO 950125 Piano Tavola, Belpasso, Italy;3. Istituto Nazionale di Biostrutture e Biosistemi (INBB), Rome, Italy |
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Abstract: | Opuntia ficus-indica L. is known for its beneficial effects on human health, but still little is known on cladodes as a potent source of antioxidants. Here, a direct, economic and safe method was set up to obtain water extracts from Opuntia ficus-indica cladodes rich in antioxidant compounds. When human keratinocytes were pre-treated with the extract before being exposed to UVA radiations, a clear protective effect against UVA-induced stress was evidenced, as indicated by the inhibition of stress-induced processes, such as free radicals production, lipid peroxidation and GSH depletion. Moreover, a clear protective effect against apoptosis in pre-treated irradiated cells was evidenced. We found that eucomic and piscidic acids were responsible for the anti-oxidative stress action of cladode extract. In conclusion, a bioactive, safe, low-cost and high value-added extract from Opuntia cladodes was obtained to be used for skin health/protection. |
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Keywords: | ABTS 2 2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) CASP-3 caspase-3 CASP-7 caspase-7 DCF 2′ 7′-dichlorofluorescein DTNB 5 5′-dithiobis-2-nitrobenzoic acid HAA hydrophilic antioxidant activity 2′ 7′-dichlorodihydrofluorescein diacetate MTT 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide ORE OSMF P-p38 phosphorylated p38 MAP kinase P-MAPKAPK-2 phosphorylated MAP kinase-activated protein kinase ROS reactive oxygen species SPF sun protection factor TEAC Trolox equivalent antioxidant capacity TBA thiobarbituric acid TBARS TBA reactive substances TNB 5-thio-2-nitrobenzoic acid Cladode water extracts Antioxidants UVA-induced oxidative stress Eucomic acid Piscidic acid |
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