Quantitative bioluminescence imaging of tumor-targeting bacteria in living animals |
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Authors: | Min Jung-Joon Nguyen Vu H Kim Hyun-Ju Hong Yeongjin Choy Hyon E |
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Institution: | Laboratory of In Vivo Molecular Imaging, Department of Nuclear Medicine, Chonnam National University Medical School, 5 Hak1 dong, Dong-gu, Gwangju 501-746, Republic of Korea. jjmin@jnu.ac.kr |
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Abstract: | We describe a protocol for imaging bacterial luciferase (Lux)-expressing bacteria in small living animals. In this protocol, light emitted by Lux-expressing bacteria is detected and monitored by a cooled charge-coupled device detector. When these bacteria are administered to animals, it provides a potentially valuable approach to generate sensitive whole-body images with extremely low background. This imaging technology should enable the real-time monitoring of bacterial migration into both primary and metastatic tumors in several different mouse tumor models at a strong quantification power. |
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