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| 植物Tnt1反转录转座子突变体库的特征及应用 | |
| 引用本文： | 魏解冰,狄少康,文江祁,庞永珍.植物Tnt1反转录转座子突变体库的特征及应用[J].植物学报,2016,51(6):817-826. |
| 作者姓名： | 魏解冰 狄少康 文江祁 庞永珍 |
| 作者单位： | 1中国科学院植物研究所北方资源植物重点实验室/北京植物园, 北京 100093; 2中国科学院大学, 北京 100049 3美国赛缪尔·诺贝尔基金会, 阿德莫尔 73402 |
| 基金项目： | 国家重点基础研究发展计划(No.2014CB138703)和国家国际科技合作专项(No.2015DFR30570) |
| 摘 要： | 植物反转录转座子是转座子中的一大类。目前, 在构建突变体库中应用较多的反转录转座子包括Tnt1、Tos17、Tto1和LORE1。该文比较了利用不同反转录转座子构建的植物突变体库的特征, 并对目前应用较为广泛的Tnt1突变体库进行了详细的分析和总结, 重点对豆科模式植物蒺藜苜蓿(Medicago truncatula) Tnt1突变体库的构建及其在固氮、代谢和发育等方面的应用进行了系统概括。该综述将有助于全面了解蒺藜苜蓿Tnt1突变体库的特征, 并为豆科植物功能基因组学研究提供有益的参考。 |
| 收稿时间： | 2016-03-08 |
| 修稿时间： | 2016-05-17 |
The Application of Retrotransposon Tnt1 in Establishing a Plant Mutant Population |
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| Jiebing Wei,Shaokang Di,Jiangqi Wen,Yongzhen Pang.The Application of Retrotransposon Tnt1 in Establishing a Plant Mutant Population[J].Bulletin of Botany,2016,51(6):817-826. | |
| Authors: | Jiebing Wei Shaokang Di Jiangqi Wen Yongzhen Pang |
| Institution: | 1Key Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; 2University of Chinese Academy of Sciences, Beijing 100049, China; 3The Samuel Roberts Noble Foundation, Ardmore 73402, USA |
| Abstract: | Plant retrotransposons are among the largest transposon families. Tnt1, Tos17, Tto1 and LORE1 have been widely used in establishing mutant populations. In the present study, we summarized and compared various approaches for establishing mutant populations, especially Tnt1 retrotransposons. We focused on advances in establishing and using the Medicago truncatula Tnt1 mutant population, including nitrogen fixation, metabolism and development. Our study will benefit the comprehensive understanding and use of the M. truncatula Tnt1 mutant population and provide valuable references for researchers studying legume functional genomes. |
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