一种快速定量分析药用植物淫羊藿生物碱的方法

建立了基于近红外漫反射光谱的药用植物淫羊藿(Epimedium)生物碱的定量分析方法,测定了9种淫羊藿生物碱的含量。当聚类分析采用主成分分析、数学处理采用2,4,4,1模式、光谱散射校正采用标准正则变换+散射处理及回归分析采用改进最小二乘法时,淫羊藿生物碱近红外定标模型的定量效果最好;定标方程的定标决定系数、交互验证相关系数、定标标准差和交互验证标准差分别为0.899 7、0.845 7、0.168 6和0.085 3。近红外定标模型预测值与常规化学分析值差异很小。应用近红外漫反射光谱法测得9种淫羊藿生物碱的含量介于6.537–20.586 mg·g–1(干重)之间,淫羊藿生物碱的种间差异较大。近红外漫反射光谱法具有快速、简便和无损(不损耗受检材料)的优点,可用于淫羊藿生物碱的研究与开发。

A Rapid Method for Quantitative Analysis of Alkaloids in Epimedium

A novel method for quantitative analysis of alkaloids in Epimedium was developed based on near-infrared reflectance spectroscopy. Content of alkaloids in nine species of Epimedium was quantified by the technique. Principal component analysis, ‘2,4,4,1’ mathematical treatment, standard normal variant and de-trending and modified partial least-squares analyses were used to establish the near-infrared model for quantifying alkaloids in Epimedium. The developed model of Epimedium alkaloids based on near-infrared spectroscopy possessed powerful predictive ability. The coefficient of determination for calibration, 1 minus variance ratio, standard error of calibration and standard error of cross-validation of calibration equation were 0.899 7, 0.845 7, 0.168 6 and 0.085 3, respectively. Alkaloid levels did not differ on analysis by the near-infrared reflectance spectroscopy method and conventional analytical method. Content of alkaloids in Epimedium samples ranged from 6.537 to 20.586 mg·g^-1 dry weight. Alkaloid content varied greatly among the different Epimedium species. The proposed method based upon near-infrared spectroscopy is a fast, convenient and nondestructive alternative to the traditional analytical method and shows great potential in research and development of alkaloids in Epimedium.