水稻响应缺铁的韧皮部汁液蛋白质组学分析

为鉴定水稻(Oryza sativa)响应缺铁的根冠长距离信号转导物质, 采用TMT标记技术分析了不同浓度铁处理下水稻韧皮部汁液的蛋白质组学变化, 共鉴定出206个差异蛋白, 其中54个蛋白表达丰度上调, 152个蛋白表达丰度下调。差异蛋白的KEGG通路分类主要包括激素信号代谢、谷胱甘肽代谢、碳代谢以及mRNA转运等代谢途径。此外, 对差异蛋白对应的生理指标进行测定, 发现激素、蔗糖、谷胱甘肽和转运蛋白等在缺铁条件下变化显著, 后续对这些差异蛋白的功能研究有助于揭示水稻响应铁素营养的长距离信号途径。

Effect of Iron Deficiency on the Protein Profile of Rice (Oryza sativa) Phloem Sap

In order to identify long-distance signals under iron (Fe) deficiency in rice (Oryza sativa), TMT label technique was used to study the protein profile of phloem sap under different iron concentrations. A total of 206 differentially ex- pressed proteins were identified: 54 were upregulated and 152 were downregulated. Most of these proteins are involved in hormone signal transduction, carbon metabolism, glutathione metabolism and mRNA transport. In addition, we mea- sured the physiological indicators according to the differentially expressed proteins. It was found that phytohormones, sucrose, glutathione and transporters were significantly changed under iron deficiency. To further study the function of these proteins can help to reveal the long distance signaling pathway of rice in response to Fe deficiency.