Microbial dehalogenation of trichloroacetic acid |
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Authors: | A. L. Weightman A. J. Weightman J. H. Slater |
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Affiliation: | (1) School of Pure and Applied Biology, University of Wales, Cardiff, PO Box 915, CF1 3TL Cardiff, UK |
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Abstract: | A pure bacterial culture and a two-membered mixed culture were isolated that degraded trichloroacetic acid if a second, readily metabolizable substrate was present in the growth medium. Previous doubts over the microbial dehalogenation of trichloroacetic acid (TCA) may be due to its inability to act as a sole carbon and energy source. TCA dehalogenation was associated with conventional 2-haloalkanoic acid dehalogenases but oxalate, the putative dehalogenase product, was not detected. CO2 was produced rapidly and concomitantly with Cl– ion release during dehalogenation of TCA. An alternative mechanism is suggested for TCA dehalogenation via an initial decarboxylation reaction. This mechanism predicts that carbon monoxide is a product of TCA decarboxylation and it was significant that one of the organisms isolated,Pseudomonas carboxydohydrogens, was a carboxytroph and a second was an unidentified facultative methylotroph. |
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Keywords: | Dehalogenases dehalogenation methylotrophs Pseudomonas trichloroacetic acid |
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