A critical study of the measurement and calibration of circular dichroism |
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| Authors: | Katura Tuzimura Toshio Konno Hiroshi Meguro Masahiro Hatano Tasuku Murakami Kazuo Kashiwabara Kazuo Saito Yoshikazu Kondo Toshishige M. Suzuki |
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| Affiliation: | 1. Department of Food Chemistry, Faculty of Agriculture, Tohoku University, Sendai, Japan;2. Chemical Research Institute of Non-Aqueous Solutions, Tohoku University, Sendai, Japan;3. Chemistry Department, Faculty of Science, Tohoku University, Sendai, Japan;4. Pharmaceutical Institute, Tohoku University, Sendai, Japan;5. Government Industrial Research Institute, Tohoku, Sendai, Japan |
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| Abstract: | A critical study has been made of circular dichroism (CD) measurements at the wavelengths around 500, 290, and 220 nm. Instruments calibrated at 290 nm with (+)-camphor-10-sulfonic acid gave results for molecular ellipticities [θ] showing deviations up to >30%, at both 220 and 490 nm. Older instruments tended to given higher values of [θ] at 490 nm and lower values at 220 nm, probably due to the age of the Pockel cells. |
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