A rapid method for separation and assay of radiolabeled mucopolysaccharides from cell culture medium |
| |
| Authors: | Heikki SaarniMarkku Tammi |
| |
| Affiliation: | Department of Medical Chemistry, University of Turku, Kiinamyllynk, 10, SF-20520 Turku 52, Finland |
| |
| Abstract: | A method is described for the separation and assay of radiolabeled glycosaminoglycuronans (GAGs) from cell culture medium. Following papain digestion and precipitation with cetylpyridinium chloride onto cellulose ester filter membrane, hyaluronic acid can be separated from sulfated GAGs by selective elution with HCl. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
