A simplified gel electrophoretic system and its validity for molecular weight determinations of protein-cetyltrimethylammonium complexes |
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| Authors: | Sakol PanyimRangsan Thitipongpanich Daungrudee Supatimusro |
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| Institution: | Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand |
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| Abstract: | Proteins ranging in molecular weight from 90,000 to 15,000 daltons were studied by polyacrylamide gel electrophoresis in the presence of cetyltrimethylammonium bromide (CTAB) at pH 4.6. The results showed a linear relationship between log molecular weight and relative electrophoretic mobility. The simplicity and reliability of the method offer an alternative means of determination of molecular weights of a wide variety of proteins by gel electrophoresis. |
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