A sensitive,fluorometric method for the assay of microsomal hydroxylase: N-demethylation of p-chloro-N-methylaniline |
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| Authors: | Th. van der Hoeven |
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| Affiliation: | Department of Medicinal Chemistry, School of Pharmacy, University of Maryland, Baltimore, Maryland, USA |
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| Abstract: | A fluorometric method for the assay of microsomal hydroxylase activity is described. N-Demethylation of p-chloro-N-methylaniline yields p-chloroaniline, which is coupled with fluorescamine, extracted with ethylacetate, and measured fluorometrically. This method can determine low levels of N-demethylase activity. |
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