Low-pressure,microwave-induced plasma spectrometry method for the determination of subnanogram quantities of mercury: Application to mercury-carboxypeptidase analysis |
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| Authors: | Ikuo Atsuya Hiroshi Kawaguchi Bert L Vallee |
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| Institution: | 1. Biophysics Research Laboratory, Department of Biological Chemistry, Harvard Medical School, Boston, Massachusetts 02115 USA;2. the Division of Medical Biology, Peter Bent Brigham Hospital, Boston, Massachusetts 02115 USA |
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| Abstract: | A simple, sensitive, and rapid method for the determination of subnanogram quantities of mercury has been devised using a tantalum-filament vaporization system and low-pressure, microwave-induced emission spectrometry. This method is applied to the analysis of mercury-substituted carboxypeptidase with a sample volume of 5 μl and an analysis time of 3 min. The coefficient of variation for 2 × 10−10 g of mercury in mercury-carboxypeptidase is 6.0%, and the detection limit is 3 × 10−12 g. |
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