| 里氏木霉306产t-PA固态发酵条件的研究 |
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| 引用本文： | 江洁,杜连祥,路福平,廖海印. 里氏木霉306产t-PA固态发酵条件的研究[J]. 工业微生物, 2005, 35(2): 14-18 |
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| 作者姓名： | 江洁 杜连祥 路福平 廖海印 |
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| 作者单位： | 天津科技大学食品科学与生物工程学院,天津,300222;齐齐哈尔大学生命科学与工程学院,齐齐哈尔,161006;天津科技大学食品科学与生物工程学院,天津,300222;齐齐哈尔大学生命科学与工程学院,齐齐哈尔,161006 |
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| 基金项目： | 黑龙江省自然科学基金资助项目(No.D0352) |
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| 摘 要： | 对基因工程菌株里氏木霉(Trichodermareesei)306生物合成组织型纤溶酶原激活剂(t-PA)的固态发酵培养基成分和发酵条件进行了研究;分析了发酵过程中t-PA的生成、总糖的消耗和菌体生长的规律。在优化的固体发酵条件下,t-PA的最大酶活是924.63IU/g干重培养基,较初始条件下提高了5倍。通过浅盘进行了放大实验,最大酶活为983.64IU/g干重培养基,t-PA合成速率为:13.66IU/g干重培养基.h,较三角瓶固态发酵最高产酶提高了6.38%,产酶速率提高了24.07%。
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| 关 键 词： | 里氏木霉 组织型纤溶酶原激活剂 固态发酵 |
Solid-state fermentation conditions for t-PA production by Trichoderma reesei 306 |
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| GANG Jie,DU Lian-xiang,LU Fu-ping,LIAO Hai-ying. Solid-state fermentation conditions for t-PA production by Trichoderma reesei 306[J]. Industrial Microbiology, 2005, 35(2): 14-18 |
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| Authors: | GANG Jie DU Lian-xiang LU Fu-ping LIAO Hai-ying |
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| Abstract: | Solid-state fermentation conditions of recombinant Trichoderma reesei 306 for t-PA production were investigated. The optimum medium and the optimum fermentation conditions were determined .Under optimized conditions ,the maximum t-PA productivity was enhanced by 5 times than that under the original fermentation conditions.The optimized technology was scaled up in the tray cultivation and the maximum t-PA productivity was 983.64IU/g dry medium, the yield was 13.66 IU/g dry mediumh, increased by 6.38% and 24.07% compared with those of flask cultivation, respectively. |
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| Keywords: | Trichoderma reesei tissue-type plasminogen activator solid-state fermentation |
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