Stabilization of a miniderivative of the broad-host-range IncW plasmid pSa by insertion of plasmid R1parB region |
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Authors: | J. Nešvera J. Hochmannová M. Pátek |
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Affiliation: | (1) Department of Molecular Genetics of Bacteria, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20 Prague 4, Czechoslovakia |
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Abstract: | The plasmid vector pEM100 (13.5 kb) constructed from pGV1106, a miniderivative of the broad-host-range IncW pSa plasmid, and the pAM330 plasmid ofBrevibacterium lactofermentum is not stably maintained inEscherichia coli host cells under nonselective growth conditions. By insertion of a 0.9 kb DNA fragment containing theparB locus (responsible for the maintenance of plasmid R1 inE. coli cells) to plasmid pEM100, plasmid pEM110 was prepared which is maintained in a population ofE. coli cells growing without a selection pressure very stably. Translated by Č. Novotny |
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