Analysis of xanthophore and pterinosome biogenesis in zebrafish using methylene blue and pteridine autofluorescence |
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Authors: | Le Guyader Sylvie Jesuthasan Suresh |
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Institution: | Fish Developmental Genetics Laboratory, Institute of Molecular Agrobiology, Singapore. |
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Abstract: | We have identified two simple methods to analyse xanthophore and pterinosome biogenesis in zebrafish. The first uses methylene blue (methylthionium chloride), a redox dye which specifically labels xanthophores and pterinosomes, while the second uses autofluorescence to detect pteridine levels; these methods may be used to detect the number, location and shape of xanthophores and pterinosomes. These assays were applied to two zebrafish mutants--brie and yobo--and revealed that both mutants have pterinosome biogenesis and pteridine synthesis defects. Additionally, using capillary electrophoresis, we provide evidence that sepiapterin is responsible for the yellow colour and blue-light induced fluorescence in zebrafish embryos. |
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