| Abstract: | Previous studies on the lipase-mediated acylation of 6-azauridine with vinyl stearate in organic solvents revealed that while
preparing a potential prodrug, 3′-O-stearoyl-6-azauridine, a lipase from Burkholderia cepacia showed high regioselectivity toward the second hydroxyl group. The most suitable reaction solvent, molar ratio of vinyl stearate
to 6-azauridine, and reaction temperature were anhydrous acetone, 15:1, and 45°C, respectively. Under these conditions, the
initial reaction rate, 3′-regioselectivity, and maximum substrate conversion were as high as 10.4 mM/h, 86.0, and 99.0%, respectively. |
