Quantification of airborne Aspergillus fumigatus spores in rabbit houses by real-time polymerase chain reaction |
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Authors: | Zengmin Miao Yumei Cai Jingbo Liu Daijun Li Tongjie Chai |
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Institution: | (1) College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an, Shandong Province, China;(2) Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Tai’an, Shandong Province, China; |
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Abstract: | To accurately quantify airborne Aspergillus fumigatus (A. fumigatus) spores in rabbit houses, the real-time polymerase chain reaction (real-time PCR) and culture-based counting method (CCM)
were employed to determine the airborne A. fumigatus spore concentrations. The results showed that, of the three rabbit houses (A, B, and C), the average concentrations of airborne
A. fumigatus spores determined by real-time PCR were 3.0 × 103, 3.3 × 103, and 1.5 × 103 spores/m3 air, respectively, while those determined by CCM were 2.5 × 102, 2.8 × 102, and 1.1 × 102 colony-forming unit/m3 air (CFU/m3 air), respectively, i.e., the former concentration was 12–14 times higher than the latter one. Therefore, the conventional
CCM underestimated the concentrations of airborne fungal spores, and it is insufficient to determine the microbial aerosol
concentration and evaluate the health risk only using CCM. |
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