| 胰腺应激蛋白PSP/reg抑制胰腺星状细胞TIMPs:MMPs及RECK表达 |
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| 引用本文： | 李玲,孙子林,李凤飞. 胰腺应激蛋白PSP/reg抑制胰腺星状细胞TIMPs:MMPs及RECK表达[J]. 中国微生态学杂志, 2011, 23(12): 1077-1081 |
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| 作者姓名： | 李玲 孙子林 李凤飞 |
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| 作者单位： | 东南大学附属中大医院内分泌科,江苏南京,210009 |
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| 基金项目： | 国家自然科学基金项目(30900696;30971399;81170716); 江苏省自然科学基金项目(BK2009278) |
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| 摘 要： | 目的 观察胰腺应激蛋白PSP/reg对胰腺星状细胞(PSC)合成和分泌基质金属蛋白酶(MMPs)及其组织抑制剂(TIMPs)以及RECK表达的影响.方法 分离纯化慢性胰腺炎患者纤维化区的PSC,基因重组胰腺应激蛋白PSP/reg,以终浓度为10和100 ng/mL对PSC进行干预,实时荧光定量PCR检测MMP1/2、TIMP1/2及RECK基因表达,Western blot测定MMP1/2、TIMP1/2及RECK蛋白,细胞免疫荧光观察细胞膜表面RECK分布.结果 PSP/reg对MMP1/2、TIMP1/2及RECK表达无明显影响；PSP/reg轻度抑制PSC培养上清中MMP2水平(P＜0.05),而显著抑制TIMP1/2水平(P ＜0.01)；PSC细胞膜表面发现有RECK蛋白,PSP/reg减少PSC的RECK含量(P＜0.01).结论 胰腺应激蛋白PSP/reg能够降低TIMPs:MMPs比率、减少RECK蛋白水平表达,从而解除对MMPs的部分抑制,使MMPs活性相对增高,有利于纤维化的分解消散,促进胰腺损伤后的再生修复.
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| 关 键 词： | PSP/reg 胰腺星状细胞 基质金属蛋白酶 组织抑制剂 RECK |
Pancreatic stress protein PSP/reg inhibits pancreatic stellate cell TIMPs:MMPs secretion and RECK expression |
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| LI Ling , SUN Zi-lin , LI Feng-fei. Pancreatic stress protein PSP/reg inhibits pancreatic stellate cell TIMPs:MMPs secretion and RECK expression[J]. Chinese Journal of Microecology, 2011, 23(12): 1077-1081 |
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| Authors: | LI Ling SUN Zi-lin LI Feng-fei |
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| Affiliation: | LI Ling,SUN Zi-lin,LI Feng-fei(Department of Endocrinology,Zhongda Hospital,Southeast University,Nanjing 210009,China) |
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| Abstract: | Objective To observe the effects of pancreatic stress protein(PSP/reg) on TIMPs:MMPs secretion and RECK expression of pancreatic stellate cell(PSC).Method PSC were obtained by outgrowth from fibrotic human pancreas tissue.PSP/reg was expressed in the yeast Pichia pastoris.PSP/reg was added at concentration of 10 and 100 ng/mL to cultured PSC.MMP1/2,TIMP1/2 and RECK mRNA level were determined by real-time quantitative RT-PCR.Their protein level were determined by Western blot.RECK was detected on the cell su... |
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| Keywords: | PSP/reg Pancreatic stellate cell MMP TIMP RECK |
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